Evaluation of the BD Max StaphSR Assay for Rapid Identification of Staphylococcus aureus and Methicillin-Resistant S. aureus in Positive Blood Culture Broths

J Clin Microbiol. 2015 Nov;53(11):3630-2. doi: 10.1128/JCM.01922-15. Epub 2015 Aug 19.

Abstract

We evaluated the performance of the BD Max StaphSR assay for the direct detection of Staphylococcus aureus from blood culture medium. In a two-center trial, 155 blood cultures from the BD Bactec FX system and 212 from the bioMérieux BacT/Alert system were tested; 170 bottles yielded S. aureus, and all were identified correctly by the BD Max StaphSR assay. The assay required approximately 2.5 h, thus allowing rapid identification of blood cultures flagged positive.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteremia / microbiology
  • Bacterial Proteins / genetics
  • Bacteriological Techniques / methods
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / classification
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Micrococcal Nuclease / genetics
  • Penicillin-Binding Proteins / genetics
  • Reagent Kits, Diagnostic*
  • Real-Time Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology

Substances

  • Bacterial Proteins
  • Penicillin-Binding Proteins
  • Reagent Kits, Diagnostic
  • mecA protein, Staphylococcus aureus
  • nuc protein, staphylococcus
  • Micrococcal Nuclease