Spatiotemporal drug delivery using laser-generated-focused ultrasound system

Jin Di; Jinwook Kim; Quanyin Hu; Xiaoning Jiang; Zhen Gu

doi:10.1016/j.jconrel.2015.08.033

Spatiotemporal drug delivery using laser-generated-focused ultrasound system

J Control Release. 2015 Dec 28;220(Pt B):592-9. doi: 10.1016/j.jconrel.2015.08.033. Epub 2015 Aug 21.

Authors

Jin Di¹, Jinwook Kim², Quanyin Hu¹, Xiaoning Jiang³, Zhen Gu⁴

Affiliations

¹ Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
² Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA.
³ Department of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: [email protected].
⁴ Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC 27695, USA; Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: [email protected].

Abstract

Laser-generated-focused ultrasound (LGFU) holds promise for the high-precision ultrasound therapy owing to its tight focal spot, broad frequency band, and stable excitation with minimal ultrasound-induced heating. We here report the development of the LGFU as a stimulus for promoted drug release from microgels integrated with drug-loaded polymeric nanoparticles. The pulsed waves of ultrasound, generated by a carbon black/polydimethylsiloxane (PDMS)-photoacoustic lens, were introduced to trigger the drug release from alginate microgels encapsulated with drug-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. We demonstrated the antibacterial capability of this drug delivery system against Escherichia coli by the disk diffusion method, and antitumor efficacy toward the HeLa cell-derived tumor spheroids in vitro. This novel LGFU-responsive drug delivery system provides a simple and remote approach to precisely control the release of therapeutics in a spatiotemporal manner and potentially suppress detrimental effects to the surrounding tissue, such as thermal ablation.

Keywords: Drug delivery; Microgels; Nanoparticles; Photoacoustic; Stimuli responsive.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Alginates / chemistry*
Anti-Bacterial Agents / administration & dosage
Anti-Bacterial Agents / chemistry
Antibiotics, Antineoplastic / administration & dosage
Antibiotics, Antineoplastic / chemistry
Cell Proliferation / drug effects
Cell Survival / drug effects
Chemistry, Pharmaceutical
Ciprofloxacin / administration & dosage
Ciprofloxacin / chemistry
Delayed-Action Preparations
Dimethylpolysiloxanes / chemistry
Disk Diffusion Antimicrobial Tests
Doxorubicin / administration & dosage
Doxorubicin / chemistry
Drug Carriers*
Equipment Design
Escherichia coli / drug effects
Escherichia coli / growth & development
Gels
Glucuronic Acid / chemistry
HeLa Cells
Hexuronic Acids / chemistry
Humans
Kinetics
Lactic Acid / chemistry*
Lasers*
Nanoparticles
Photoacoustic Techniques
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Solubility
Soot / chemistry
Spheroids, Cellular
Technology, Pharmaceutical / instrumentation*
Technology, Pharmaceutical / methods
Transducers*
Ultrasonic Waves*
Ultrasonics / instrumentation*
Ultrasonics / methods

Substances

Alginates
Anti-Bacterial Agents
Antibiotics, Antineoplastic
Delayed-Action Preparations
Dimethylpolysiloxanes
Drug Carriers
Gels
Hexuronic Acids
Soot
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid
Ciprofloxacin
Doxorubicin
Glucuronic Acid

Abstract

Publication types

MeSH terms

Substances

Grants and funding