MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon

Xiyu Duan; Haijun Li; Zhen Qiu; Bishnu P Joshi; Asha Pant; Arlene Smith; Katsuo Kurabayashi; Kenn R Oldham; Thomas D Wang

doi:10.1364/BOE.6.003074

MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon

Biomed Opt Express. 2015 Jul 29;6(8):3074-83. doi: 10.1364/BOE.6.003074. eCollection 2015 Aug 1.

Authors

Xiyu Duan¹, Haijun Li², Zhen Qiu³, Bishnu P Joshi², Asha Pant², Arlene Smith², Katsuo Kurabayashi⁴, Kenn R Oldham⁵, Thomas D Wang⁶

Affiliations

¹ Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
² Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA.
³ Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA ; Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA.
⁴ Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA ; Department of Electrical Engineering, University of Michigan, Ann Arbor, MI, USA.
⁵ Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
⁶ Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA ; Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA ; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.

Abstract

We demonstrate a handheld multiphoton endomicroscope with 3.4 mm distal diameter that can repetitively image mouse colon in vivo. A 2D resonant MEMS mirror was developed to perform beam scanning in a Lissajous pattern. The instrument has an effective numerical aperture of 0.63, lateral and axial resolution of 2.03 and 9.02 μm, respectively, working distance of 60 μm, and image field-of-view of 300 × 300 μm(2). Hoechst was injected intravenously in mice to stain cell nuclei. We were able to collect histology-like images in vivo at 5 frames/sec, and distinguish between normal and pre-malignant colonic epithelium.

Keywords: (060.2350) Fiber optics imaging; (110.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (170.2150) Endoscopic imaging; (190.4180) Multiphoton processes.

Abstract

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