Gene probes for the detection of Listeria spp

Acta Microbiol Hung. 1989;36(2-3):211-3.

Abstract

Four cloned genes of Listeria monocytogenes coding for listeriolysin O, beta-haemolysin, CAMP-factor and a DTH-inducing protein (DTH-18) were used as gene probes in DNA-DNA hybridization with different listerial references strains. The fragment encoding for DTH-18 is the only probe reacting specifically with pathogenic strains of L. monocytogenes and Listeria ivanovii.

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics*
  • Blotting, Southern
  • DNA Probes*
  • DNA, Bacterial / genetics
  • DNA, Recombinant
  • Genes, Bacterial*
  • Hemolysin Proteins / analysis
  • Hemolysin Proteins / genetics*
  • Listeria / classification
  • Listeria / genetics
  • Listeria / isolation & purification*

Substances

  • Bacterial Proteins
  • DNA Probes
  • DNA, Bacterial
  • DNA, Recombinant
  • Hemolysin Proteins