Characterization of Carriage Isolates of Neisseria meningitides in the Adolescents and Young Adults Population of Bogota (Colombia)

PLoS One. 2015 Aug 31;10(8):e0135497. doi: 10.1371/journal.pone.0135497. eCollection 2015.

Abstract

Background: Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.

Materials and methods: A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.

Results: The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

Conclusion: The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antigens, Bacterial / immunology
  • Carrier State / microbiology*
  • Colombia
  • Female
  • Genotype
  • Humans
  • Male
  • Meningitis, Meningococcal / microbiology*
  • Neisseria meningitidis / genetics
  • Neisseria meningitidis / isolation & purification*
  • Oropharynx / microbiology
  • Prevalence
  • Serotyping / methods
  • Students
  • Young Adult

Substances

  • Antigens, Bacterial

Grants and funding

This study was supported by Sanofi-Pasteur, through the Pan-American Health and Education Foundation (PAHEF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.