Induction of Colon Cancer in Mice with 1,2-Dimethylhydrazine

Cold Spring Harb Protoc. 2015 Sep 1;2015(9):pdb.prot077453. doi: 10.1101/pdb.prot077453.

Abstract

In this protocol, colon cancer is induced in mice through a series of injections with 1,2-dimethylhydrazine. Mice will develop primarily colon tumors starting at about 3 mo after the first injection. Tumors in the lung, uterus, and small intestine may also be seen, as well as lymphomas.

MeSH terms

  • 1,2-Dimethylhydrazine / administration & dosage
  • 1,2-Dimethylhydrazine / metabolism*
  • Animals
  • Colonic Neoplasms / chemically induced*
  • Colonic Neoplasms / pathology
  • Injections, Subcutaneous
  • Mice
  • Time Factors

Substances

  • 1,2-Dimethylhydrazine