CLA and CD62E expression in oral lichen planus lesions

J Oral Pathol Med. 2016 Mar;45(3):218-23. doi: 10.1111/jop.12355. Epub 2015 Aug 31.

Abstract

Objective: There are few reports on the migration of CLA+ T cells through E-selectin in cutaneous lichen planus, with only one study on oral lichen planus (OLP). This study aimed to analyze CLA expression and assess whether there is a correlation with E-selectin (CD62E) in OLP lesions.

Material and methods: Biopsies were performed on 11 patients including two areas: one without clinical and histopathological features of OLP [perilesional group (PLG)] and the other with clinical and histopathological features of OLP [OLP group (OLPG)]. The specimens obtained were divided into two: One was fixed in formalin for routine analysis (H&E), and the other was frozen for CD3, CD4, CD8, CLA, and CD62E immunofluorescence markers.

Results: More CD4+ (median 1409, range 860-2519), CD8+ (median 1568, range 654-3258), and CLA+ T cells (median 958, range 453-2198) and higher CD62E expression (median 37, range 27-85) were identified in OLPG (P = 0.003; P = 0.003; P = 0.004; P = 0.003, respectively) than those in PLG. The median prevalence analysis was also significantly higher for CLA+CD8+ T cells in OLPG (OLPG = 39.4%, range 18.4-64.2; PLG = 29.4%, range 12.1-47.1) (P = 0.026). None of the correlations between CD3+ or CLA+ T cells and CD62E in OLPG and in PLG were significant.

Conclusion: The significant presence of CLA+ T cells and E-selectin expressions in the OLPG suggests their involvement in the etiopathogenesis of OLP; however, only a weak correlation between CLA+ T cells and E-selectin was observed.

Keywords: CD8; CLA; E-selectin; Oral lichen planus; lymphocytes; oral immunology.

MeSH terms

  • Antigens, CD / immunology
  • Antigens, CD / metabolism
  • Antigens, Differentiation, T-Lymphocyte / biosynthesis*
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Antigens, Differentiation, T-Lymphocyte / metabolism
  • E-Selectin / biosynthesis*
  • E-Selectin / immunology
  • E-Selectin / metabolism
  • Fluorescent Antibody Technique, Direct / methods
  • Humans
  • Lichen Planus / immunology
  • Lichen Planus / metabolism
  • Lichen Planus / pathology
  • Lichen Planus, Oral / immunology
  • Lichen Planus, Oral / metabolism*
  • Lichen Planus, Oral / pathology
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Lymphocytes / pathology
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism
  • Mouth Mucosa / immunology
  • Mouth Mucosa / metabolism
  • Mouth Mucosa / pathology
  • Prevalence
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CTAGE1 protein, human
  • E-Selectin
  • Membrane Glycoproteins
  • SELE protein, human