T-shaped trichome-specific expression of monoterpene synthase ADH2 using promoter-β-GUS fusion in transgenic Artemisia annua L

Biotechnol Appl Biochem. 2016 Nov;63(6):834-840. doi: 10.1002/bab.1440. Epub 2015 Nov 5.

Abstract

Artemisinin, a sesquiterpene lactone isolated from Artemisia annua L. (sweet wormwood), is extensively used in the treatment of malaria. In order to better understand the metabolism of terpenes in A. annua and the influence of terpene synthases on artemisinin yield, the expression pattern of a monoterpene alcohol dehydrogenase (ADH2) has been studied using transgenic plants expressing promoter-β-glucuronidase (GUS) fusion. ADH2 played a major role in monoterpenoid biosynthesis including carveol, borneol, and artemisia ketone through in vitro biochemical analysis. In this study, the ADH2 promoter was cloned by the genome walking method. A number of putative cis-acting elements were predicted in promoter region, suggesting that the ADH2 is driven by a complex regulation mechanism. ADH2 gene was highly expressed in old leaves, whereas the artemisinin biosynthetic genes were mainly expressed in bud and young leaves. The expression of ADH2 gene increased quickly during leaf development, revealed by qRT-PCR. GUS expression analysis in different tissues of transgenic A. annua demonstrates that ADH2 expression is exclusively located to T-shaped trichome, not glandular secretory trichome.

Keywords: Artemisia annua L; T-shaped trichome; artemisinin production; cis-acting regulatory element; glandular trichome; monoterpene synthase; promoter activity; terpenoids.

MeSH terms

  • Alkyl and Aryl Transferases / genetics*
  • Artemisia annua / cytology
  • Artemisia annua / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Regulation, Plant*
  • Genomics
  • Glucuronidase / genetics*
  • Organ Specificity
  • Plant Leaves / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics*
  • Recombinant Fusion Proteins / genetics*
  • Trichomes / genetics*

Substances

  • Recombinant Fusion Proteins
  • Alkyl and Aryl Transferases
  • terpene synthase
  • Glucuronidase