Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages

Mar Drugs. 2015 Aug 28;13(9):5593-605. doi: 10.3390/md13095593.

Abstract

Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.

Keywords: NADPH oxidase; NF-κB; inflammatory; micrometam C; zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants
  • Cell Line
  • Coumaric Acids / chemistry*
  • Coumaric Acids / pharmacology*
  • Gene Expression Regulation / drug effects
  • Inflammation / drug therapy*
  • Lipopolysaccharides / toxicity
  • Macrophages
  • Mice
  • Molecular Structure
  • NADPH Oxidases / metabolism
  • NF-kappa B / genetics
  • NF-kappa B / metabolism
  • Oxidative Stress
  • Respiratory Burst
  • Tail
  • Zebrafish

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Coumaric Acids
  • Lipopolysaccharides
  • NF-kappa B
  • micrometam C
  • NADPH Oxidases