Optimal Cutoff Titers for Indirect Immunofluorescence Assay for Diagnosis of Scrub Typhus

J Clin Microbiol. 2015 Nov;53(11):3663-6. doi: 10.1128/JCM.01680-15. Epub 2015 Sep 9.

Abstract

We determined the optimal cutoff titers in admission and convalescent-phase samples for scrub typhus indirect immunofluorescence assay using Bayesian latent class models. Cutoff titers of ≥1:3,200 in an admission sample or of a ≥4-fold rise to ≥1:3,200 in a convalescent-phase sample provided the highest accuracy (sensitivity, 81.6%; specificity, 100%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology
  • Female
  • Fluorescent Antibody Technique, Indirect / methods*
  • Humans
  • Immunoglobulin M / blood
  • Immunoglobulin M / immunology
  • Male
  • Middle Aged
  • Orientia tsutsugamushi / immunology*
  • ROC Curve
  • Scrub Typhus / diagnosis*
  • Scrub Typhus / microbiology

Substances

  • Antibodies, Bacterial
  • Immunoglobulin M