Conservational management practices in grasslands have been considered one of the efficient options to enhance the soil organic carbon (SOC) accumulation. However, the SOC changes after the conservational management practices vary significantly under different grassland vegetation types and the environmental conditions. At present, it is not clear how the SOC accumulation changes along the soil profile if conservational management practice was adopted. In this study, we collected 663 paired observational data of SOC changes with and without conservational management practices in grasslands of China from 176 published literatures that has both the surface (0‒20 cm) and subsurface (to 40 cm depth) SOC measurements. The differences of SOC density (SOCD) between pre‒management and post‒management in the vertical soil layers were analyzed in order to establish a quantitative relationship of the SOC changes between the subsurface and the surface. The results revealed that in all grasslands, conservational management practices benefits the SOC accumulation by enhancing 0.43‒1.14 Mg C ha-1 yr-1. But the SOC increment weakened downwards along the soil profile. While the surface SOC was enhanced by 17% after conservational management, the subsurface SOC was enhanced by only 7%. The SOC accumulation was closely correlated with restoration duration, pre-management SOCD and the environmental factors and differed greatly among different grasslands and the practices adopted. The alpine and mountain grassland showed a higher annual SOC increment than the temperate grassland with the annual rate of 1.62 and 0.72 Mg C ha-1 yr-1, respectively. The SOC increment caused by the artificial plantation and the grazing exclusion conservational management was more than 2-fold that of the cropland abandonment and the extensive utilization. With the quantitative relationship of the SOC changes between soil layers, we provide a methodological option to estimate SOC changes to layers deeper than the recommendation of IPCC when only the surface layer SOC increment is available.