Plasma citrulline after small bowel transplantation: effect of time from transplantation, acute cellular rejection, and renal failure

Clin Transplant. 2015 Nov;29(11):1039-46. doi: 10.1111/ctr.12630. Epub 2015 Oct 18.

Abstract

Background: Plasma citrulline concentration (CIT) depends on its synthesis by enterocytes and its catabolism by renal tubules. To evaluate CIT applicability as a marker of acute cellular rejection (ACR) after intestinal transplantation (ITx), CIT was investigated according to time from ITx, episodes of ACR, and creatinine clearance (CrCl).

Methods: Twenty-four adult ITx recipients were prospectively studied. The results were compared with those of 19 healthy controls (HCs) and of 29 patients with chronic renal failure (CRF).

Results: In ITx recipients, CIT was lower than in HCs during the first two postoperative weeks; it then progressively increased and reached the range observed in HCs, approximately between the 31st and the 45th postoperative day. A positive association with postoperative days (R = 0.63; p < 0.0001) and a negative association with CrCl (R = -0.57; p < 0.0001) were observed. CIT was higher in patients with CRF than in HCs (p < 0.0001). CIT sensitivity and specificity in detecting ACR after the 45th postoperative day were 38% and 83%, using CIT threshold observed in HCs, and 69% and 77%, respectively, using CIT threshold adjusted for CRF degree.

Conclusions: Adjusting CIT threshold for CRF degree almost doubled the sensitivity of CIT as a non-invasive marker of ACR in ITx recipients.

Keywords: acute cellular rejection; intestinal transplantation; plasma citrulline.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Biomarkers / blood*
  • Case-Control Studies
  • Citrulline / blood*
  • Female
  • Follow-Up Studies
  • Graft Rejection / blood
  • Graft Rejection / diagnosis*
  • Graft Survival
  • Humans
  • Intestinal Diseases / blood
  • Intestinal Diseases / complications
  • Intestinal Diseases / surgery*
  • Intestine, Small / transplantation*
  • Male
  • Middle Aged
  • Postoperative Complications*
  • Prognosis
  • Prospective Studies
  • Renal Insufficiency / blood
  • Renal Insufficiency / diagnosis*
  • Risk Factors
  • Time Factors
  • Young Adult

Substances

  • Biomarkers
  • Citrulline