[Interleukin-37 inhibits the motility and migration of mouse splenic dendritic cells]

Yuechen Luo; Fang Liu; Wenwen Li; Wantong Wu; Zhina Sun; Chunxiao Zhao; Yue Shen

[Interleukin-37 inhibits the motility and migration of mouse splenic dendritic cells]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Sep;31(9):1153-5, 1161.

[Article in Chinese]

Authors

Yuechen Luo¹, Fang Liu¹, Wenwen Li¹, Wantong Wu¹, Zhina Sun¹, Chunxiao Zhao¹, Yue Shen¹

Affiliation

¹ State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.

PMID: 26359090

Abstract

Objective: To investigate the effect of interleukin-37 (IL-37) on the motility and migration of mouse splenic dendritic cells (DCs).

Methods: Magnetic bead cell sorting was used to isolate mouse splenic DCs, and flow cytometry was used to analyze the purity. DCs were visualized in real time by PerkinElmer laser confocal microscopy and analyzed by Volocity image analysis software. The alteration of chemokine (C motif) receptor 1 (XCR1) mRNA was revealed by microarray analysis and confirmed by real-time PCR and flow cytometry. Migration ability of DCs was detected by Transwell(TM) migration assay.

Results: The purity of isolated DCs was over 95%. The treatment of IL-37 reduced the velocity of DCs mobility, decreased XCR1 expression and depressed the cell migration ability.

Conclusion: IL-37 can inhibit the motility and migration of mouse splenic DCs.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement / drug effects*
Dendritic Cells / cytology
Dendritic Cells / drug effects*
Dendritic Cells / physiology
Interleukin-1 / pharmacology*
Mice
Mice, Inbred C57BL
Receptors, Chemokine / analysis
Spleen / cytology

Substances

IL37 protein, human
Interleukin-1
Receptors, Chemokine
XC chemokine receptor 1, mouse