Bacterial CRISPR: accomplishments and prospects

Jason M Peters; Melanie R Silvis; Dehua Zhao; John S Hawkins; Carol A Gross; Lei S Qi

doi:10.1016/j.mib.2015.08.007

Bacterial CRISPR: accomplishments and prospects

Curr Opin Microbiol. 2015 Oct:27:121-6. doi: 10.1016/j.mib.2015.08.007. Epub 2015 Sep 10.

Authors

Jason M Peters¹, Melanie R Silvis², Dehua Zhao³, John S Hawkins⁴, Carol A Gross⁵, Lei S Qi⁶

Affiliations

¹ Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA.
² Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
³ Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Chemistry, Engineering & Medicine for Human Health (ChEM-H), Stanford University, Stanford, CA 94305, USA.
⁴ Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; Biophysics Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
⁵ Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: [email protected].
⁶ Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Chemistry, Engineering & Medicine for Human Health (ChEM-H), Stanford University, Stanford, CA 94305, USA. Electronic address: [email protected].

Abstract

In this review we briefly describe the development of CRISPR tools for genome editing and control of transcription in bacteria. We focus on the Type II CRISPR/Cas9 system, provide specific examples for use of the system, and highlight the advantages and disadvantages of CRISPR versus other techniques. We suggest potential strategies for combining CRISPR tools with high-throughput approaches to elucidate gene function in bacteria.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Bacteria / genetics*
CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
Genetic Engineering / methods*
Genome, Bacterial

Abstract

Publication types

MeSH terms

Grants and funding