TIA-1 and TIAR interact with 5'-UTR of enterovirus 71 genome and facilitate viral replication

Biochem Biophys Res Commun. 2015 Oct 16;466(2):254-9. doi: 10.1016/j.bbrc.2015.09.020. Epub 2015 Sep 9.

Abstract

Enterovirus 71 is one of the major causative pathogens of HFMD in children. Upon infection, the viral RNA is translated in an IRES-dependent manner and requires several host factors for effective replication. Here, we found that T-cell-restricted intracellular antigen 1 (TIA-1), and TIA-1 related protein (TIAR) were translocated from nucleus to cytoplasm after EV71 infection and localized to the sites of viral replication. We found that TIA-1 and TIAR can facilitate EV71 replication by enhancing the viral genome synthesis in host cells. We demonstrated that both proteins bound to the stem-loop I of 5'-UTR of viral genome and improved the stability of viral genomic RNA. Our results suggest that TIA-1 and TIAR are two new host factors that interact with 5-UTR of EV71 genome and positively regulate viral replication.

Keywords: 5′-UTR; Enterovirus 71; RNA binding proteins; TIA-1; TIAR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions*
  • Enterovirus A, Human / genetics*
  • Enterovirus A, Human / physiology*
  • Genome, Viral
  • Humans
  • Poly(A)-Binding Proteins / physiology*
  • RNA, Viral / metabolism
  • RNA-Binding Proteins / physiology*
  • T-Cell Intracellular Antigen-1
  • Virus Replication*

Substances

  • 5' Untranslated Regions
  • Poly(A)-Binding Proteins
  • RNA, Viral
  • RNA-Binding Proteins
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human
  • TIAL1 protein, human