Biochemical Characterization of VIM-39, a VIM-1-Like Metallo-β-Lactamase Variant from a Multidrug-Resistant Klebsiella pneumoniae Isolate from Greece

Antimicrob Agents Chemother. 2015 Dec;59(12):7811-4. doi: 10.1128/AAC.01935-15. Epub 2015 Sep 14.

Abstract

VIM-39, a VIM-1-like metallo-β-lactamase variant (VIM-1 Thr33Ala His224Leu) was identified in a clinical isolate of Klebsiella pneumoniae belonging to sequence type 147. VIM-39 hydrolyzed ampicillin, cephalothin, and imipenem more efficiently than did VIM-1 and VIM-26 (a VIM-1 variant with the His224Leu substitution) because of higher turnover rates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ampicillin / metabolism*
  • Ampicillin / pharmacology
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Typing Techniques
  • Base Sequence
  • Biotransformation
  • Cephalothin / metabolism*
  • Cephalothin / pharmacology
  • Gene Expression
  • Greece
  • Humans
  • Hydrolysis
  • Imipenem / metabolism*
  • Imipenem / pharmacology
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Kinetics
  • Klebsiella Infections / drug therapy
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • beta-Lactam Resistance / genetics
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Isoenzymes
  • Imipenem
  • Ampicillin
  • VIM-1 metallo-beta-lactamase
  • beta-Lactamases
  • Cephalothin

Associated data

  • GENBANK/KF131539