Prevalence of Providencia Strains among Patients with Diarrhea and in Retail Meats in Thailand

Jpn J Infect Dis. 2016 Jul 22;69(4):323-5. doi: 10.7883/yoken.JJID.2015.224. Epub 2015 Sep 11.

Abstract

In the present study, we examined the prevalence of Providencia spp. in retail meats and in stool samples from diarrheal patients in Thailand. Providencia-specific (Psp)-PCR amplification was positive for 58% (15/26) of the chicken, 64% (16/25) of the pork, and 68% (17/25) of the beef samples. Subsequently, Providencia strains were isolated from 65% (17/26) of the chicken, 68% (17/25) of the pork, and 72% (18/25) of the beef samples. Although P. alcalifaciens (40-58%) was the most prevalent Providencia strain, P. rettgeri, P. stuartii, and P. rustigianii were also isolated. Of the patient stool samples tested, 7.5% (16/214) had Psp-PCR products, of which 4 were P. alcalifaciens (1.9%), 4 were P. rettgeri (1.9%), and 1 was P. stuartii (0.5%). In conclusion, retail meats were substantially contaminated with Providencia, especially P. alcalifaciens. Therefore, retail meats are possibly the source of Providencia infections in humans.

MeSH terms

  • Animals
  • Cattle
  • Chickens
  • DNA, Bacterial / genetics*
  • Diarrhea / diagnosis
  • Diarrhea / epidemiology*
  • Diarrhea / microbiology
  • Enterobacteriaceae Infections / diagnosis
  • Enterobacteriaceae Infections / epidemiology*
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / transmission
  • Feces / microbiology
  • Meat / microbiology*
  • Polymerase Chain Reaction
  • Prevalence
  • Providencia / genetics*
  • Providencia / isolation & purification
  • Swine
  • Thailand / epidemiology

Substances

  • DNA, Bacterial