Autoradiographic and histopathological studies of boric acid-mediated BNCT in hepatic VX2 tumor-bearing rabbits: Specific boron retention and damage in tumor and tumor vessels

C H Yang; Y T Lin; Y H Hung; J W Liao; J J Peir; H M Liu; Y L Lin; Y M Liu; Y W Chen; K S Chuang; F I Chou

doi:10.1016/j.apradiso.2015.08.034

Autoradiographic and histopathological studies of boric acid-mediated BNCT in hepatic VX2 tumor-bearing rabbits: Specific boron retention and damage in tumor and tumor vessels

Appl Radiat Isot. 2015 Dec:106:176-80. doi: 10.1016/j.apradiso.2015.08.034. Epub 2015 Aug 28.

Authors

C H Yang¹, Y T Lin², Y H Hung³, J W Liao⁴, J J Peir², H M Liu², Y L Lin⁵, Y M Liu⁶, Y W Chen⁶, K S Chuang⁷, F I Chou⁸

Affiliations

¹ Department of Biomedical Engineering & Environmental Sciences, National Tsing Hua University, Taiwan, ROC; Department of Imaging Diagnosis, Taoyuan General Hospital, Ministry of Health and Welfare, Taiwan, ROC.
² Nuclear Science and Technology Development Center, National Tsing Hua University, Taiwan, ROC.
³ Institute of Nuclear Engineering and Science, National Tsing Hua University, Taiwan, ROC.
⁴ Graduate Institute of Veterinary Pathobiology, National Chung Hsing University, Taiwan, ROC.
⁵ Department of Radiology, Mackay Memorial Hospital, Hsinchu, Taiwan, ROC.
⁶ Department of Oncology, Taipei Veterans General Hospital, Taiwan, ROC.
⁷ Department of Biomedical Engineering & Environmental Sciences, National Tsing Hua University, Taiwan, ROC.
⁸ Nuclear Science and Technology Development Center, National Tsing Hua University, Taiwan, ROC; Institute of Nuclear Engineering and Science, National Tsing Hua University, Taiwan, ROC.

PMID: 26372198
DOI: 10.1016/j.apradiso.2015.08.034

Abstract

Hepatoma is a malignant tumor that responds poorly to conventional therapies. Boron neutron capture therapy (BNCT) may provide a better way for hepatoma therapy. In this research, (10)B-enriched boric acid (BA, 99% (10)B) was used as the boron drug. A multifocal hepatic VX2 tumor-bearing rabbit model was used to study the mechanisms of BA-mediated BNCT. Autoradiography demonstrated that BA was selectively targeted to tumors and tumor vessels. Histopathological examination revealed the radiation damage to tumor-bearing liver was concentrated in the tumor regions during BNCT treatment. The selective killing of tumor cells and the destruction of the blood vessels in tumor masses may be responsible for the success of BA-mediated BNCT for liver tumors.

Keywords: Autoradiography; Boric acid-mediated BNCT; Hepatoma; Histopathological observation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoradiography
Boric Acids / chemistry*
Boron Neutron Capture Therapy*
Liver Neoplasms, Experimental / blood supply
Liver Neoplasms, Experimental / pathology
Liver Neoplasms, Experimental / radiotherapy*
Rabbits

Substances

Boric Acids
boric acid