Abstract
Background:
Neonatal and post-weaning colibacillosis caused by enterotoxigenic E. coli is responsible for substantial economic losses encountered by the pork industry. Intestinal colonization of young piglets by E. coli depends on the efficiency of bacterial attachment to host gastrointestinal epithelium that is mediated by fimbriae. We tested the effect of porcine individual milk fat globule membrane (MFGM) proteins on F4ac positive E. coli attachment to porcine enterocytes in vitro.
Results:
Butyrophilin, lactadherin and fatty acid binding protein inhibited fimbriae-dependent adherence of E. coli to enterocytes in vitro, while xanthine dehydrogenase did not. The inhibiting activity was dose-dependent for all three proteins, but the inhibiting efficiency was different.
Conclusions:
The results indicate that MFGM proteins may interfere with attachment of E. coli to porcine neonatal intestinal mucosa.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Bacterial / genetics
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Antigens, Bacterial / metabolism*
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Bacterial Adhesion / drug effects*
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Butyrophilins
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Cell Line
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Enterocytes
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Enterotoxigenic Escherichia coli / drug effects
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Enterotoxigenic Escherichia coli / physiology*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Fatty Acid-Binding Proteins / administration & dosage
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Fatty Acid-Binding Proteins / pharmacology*
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Fimbriae Proteins / genetics
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Fimbriae Proteins / metabolism*
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Membrane Glycoproteins / administration & dosage
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Membrane Glycoproteins / pharmacology*
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Milk Proteins / administration & dosage
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Milk Proteins / pharmacology*
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Swine
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Xanthine Dehydrogenase / administration & dosage
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Xanthine Dehydrogenase / pharmacology*
Substances
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Antigens, Bacterial
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Butyrophilins
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Escherichia coli Proteins
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Fatty Acid-Binding Proteins
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K88 antigen, E coli
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Membrane Glycoproteins
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Milk Proteins
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Fimbriae Proteins
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Xanthine Dehydrogenase