Abstract
Quantitative cysteine-independent ligation of a Gd(3+) tag to genetically encoded p-azido-L-phenylalanine via Cu(I)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd(3+)-Gd(3+) distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd(3+) ion relative to the protein can be predicted with high accuracy.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Azides / chemistry
-
Carrier Proteins / chemical synthesis*
-
Carrier Proteins / chemistry
-
Click Chemistry
-
Electron Spin Resonance Spectroscopy*
-
Escherichia coli / chemistry*
-
Gadolinium*
-
Glutamates / chemistry*
-
Mutagenesis, Site-Directed
-
Phenylalanine / analogs & derivatives*
-
Phenylalanine / chemistry
-
Phenylalanine / genetics
-
Protein Structure, Tertiary*
-
Spin Labels
Substances
-
Azides
-
Carrier Proteins
-
Glutamates
-
Spin Labels
-
4-azidophenylalanine
-
Phenylalanine
-
Gadolinium