Suppression of ITGB4 Gene Expression in PC-3 Cells with Short Interfering RNA Induces Changes in the Expression of β-Integrins Associated with RGD-Receptors

E N Knyazev; K M Nyushko; B Ya Alekseev; T R Samatov; M Yu Shkurnikov

doi:10.1007/s10517-015-3011-9

Suppression of ITGB4 Gene Expression in PC-3 Cells with Short Interfering RNA Induces Changes in the Expression of β-Integrins Associated with RGD-Receptors

Bull Exp Biol Med. 2015 Aug;159(4):541-5. doi: 10.1007/s10517-015-3011-9. Epub 2015 Sep 22.

Authors

E N Knyazev¹, K M Nyushko², B Ya Alekseev², T R Samatov¹, M Yu Shkurnikov^{3

4}

Affiliations

¹ BioClinicum Research and Technical Centre, Moscow, Russia.
² Federal Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
³ BioClinicum Research and Technical Centre, Moscow, Russia. [email protected].
⁴ Federal Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. [email protected].

PMID: 26395630
DOI: 10.1007/s10517-015-3011-9

Abstract

We studied the effect of transfection of PC-3 prostate cancer cells with a plasmid encoding shRNA complimentary to a fragment of integrin β4 (ITGB4). The results attest to considerable changes in the transcriptome of transfected cells. For instance, compensatory changes in the expression of integrin family genes were found.

Keywords: ITGB4; RNA interference; integrin; metastasizing; prostate cancer.

MeSH terms

Animals
Cell Line, Tumor
Gene Expression
Gene Knockdown Techniques
Humans
Integrin beta Chains / genetics
Integrin beta Chains / metabolism
Integrin beta4 / genetics
Integrin beta4 / metabolism*
Male
Mice
Neoplasm Transplantation
Prostatic Neoplasms
RNA Interference
RNA, Small Interfering / genetics
Receptors, Immunologic / metabolism*
Receptors, Peptide / metabolism*

Substances

ITGB4 protein, human
Integrin beta Chains
Integrin beta4
RNA, Small Interfering
Receptors, Immunologic
Receptors, Peptide
arginyl-glycyl-aspartic acid directed cell adhesion receptor