Cross-presentation of cutaneous melanoma antigen by migratory XCR1⁺CD103^- and XCR1⁺CD103⁺ dendritic cells

The question of which dendritic cells (DCs) cross-present peripheral tumor antigens remains unanswered. We assessed the ability of multiple skin-derived and lymphoid resident DCs to perform this function in a novel orthotopic murine melanoma model where tumor establishment and expansion is within the skin. Two migratory populations defined as CD103^-XCR1⁺ and CD103⁺XCR1⁺ efficiently cross-presented melanoma-derived antigen, with the CD103^-XCR1⁺ DCs surprisingly dominating this process. These results are critical for understanding how antitumor CD8⁺ T cell immunity is coordinated to tumor antigens present within the skin.