Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease

Chemistry. 2015 Nov 9;21(46):16359-63. doi: 10.1002/chem.201502934. Epub 2015 Sep 30.

Abstract

A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.

Keywords: DNA polymerase; aptamer; biosensors; infectious disease; nucleic acid testing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Biosensing Techniques / methods*
  • Communicable Diseases / diagnosis*
  • Cost-Benefit Analysis
  • DNA-Directed DNA Polymerase / chemistry*
  • DNA-Directed DNA Polymerase / metabolism
  • Fluorescence Polarization
  • Fluorescent Dyes / chemistry*
  • Nucleic Acid Amplification Techniques / economics*
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acids / analysis
  • Nucleic Acids / chemistry*
  • Nucleic Acids / metabolism
  • Salmonella / chemistry*

Substances

  • Fluorescent Dyes
  • Nucleic Acids
  • DNA-Directed DNA Polymerase