Abstract
Klebsiella pneumoniae mutants were obtained after UV irradiation and negative selection with anticapsular serum. Unencapsulation, rather than expression of a structurally altered capsule, was found in the mutants. The mutant strains showed no alterations in their outer membrane proteins and lipopolysaccharide, and a great similarity with the wild type in the properties tested (serum resistance, antimicrobial sensitivity, and lipopolysaccharide-specific bacteriophage sensitivity), with the exception of a higher cell surface hydrophobicity and resistance to bacteriophage FC3-9.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Outer Membrane Proteins / analysis
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Bacteriophages
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Cell Membrane / analysis
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / isolation & purification*
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Klebsiella pneumoniae / pathogenicity
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Klebsiella pneumoniae / ultrastructure
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Lipopolysaccharides / analysis
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Microscopy, Electron
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Mutation
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Polysaccharides, Bacterial / physiology*
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Ultraviolet Rays
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Virulence
Substances
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Bacterial Outer Membrane Proteins
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Lipopolysaccharides
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Polysaccharides, Bacterial