Abstract
It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cross-Linking Reagents / chemistry
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Electrophoresis, Polyacrylamide Gel
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Exosomes / chemistry
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Green Fluorescent Proteins / genetics
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Liver / chemistry
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Mass Spectrometry / methods*
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Mice, Transgenic
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Models, Molecular*
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Multiprotein Complexes / chemistry*
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Multiprotein Complexes / genetics
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Protein Conformation*
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae Proteins / chemistry
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Sensitivity and Specificity
Substances
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Cross-Linking Reagents
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Multiprotein Complexes
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins