Beyond the Linear Genome: Paired-End Sequencing as a Biophysical Tool

Viviana I Risca; William J Greenleaf

doi:10.1016/j.tcb.2015.08.004

Beyond the Linear Genome: Paired-End Sequencing as a Biophysical Tool

Trends Cell Biol. 2015 Dec;25(12):716-719. doi: 10.1016/j.tcb.2015.08.004. Epub 2015 Oct 1.

Authors

Viviana I Risca¹, William J Greenleaf²

Affiliations

¹ Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
² Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA; Department of Applied Physics, Stanford University, Stanford, CA, 94305, USA. Electronic address: [email protected].

Abstract

Paired-end sequencing has enabled a variety of new methods for high-throughput interrogation of both genome structure and chromatin architecture. Here, we discuss how the paired-end paradigm can be used to interpret sequencing data as biophysical measurements of in vivo chromatin structure that report on single molecules in single cells.

Keywords: chromatin; chromosome structure; in vivo measurements; paired-end sequencing.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biophysical Phenomena / genetics*
Chromosome Mapping / methods*
Chromosome Mapping / trends
Genome / genetics*
Humans
Sequence Analysis, DNA / methods*
Sequence Analysis, DNA / trends

Abstract

Publication types

MeSH terms

Grants and funding