Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta

Methods Mol Biol. 2015:1339:387-94. doi: 10.1007/978-1-4939-2929-0_29.

Abstract

Plaque development has been extensively studied using magnetic resonance imaging (MRI) in animal models of rapidly progressing atherosclerosis, such as apolipoprotein E-knockout (apoE-KO) mice. Preclinical MRI plays a significant role in the study of experimental atherosclerosis. Currently, MRI is capable of detecting luminal narrowing, plaque size, and morphology with high accuracy and reproducibility, providing reliable measurements of plaque burden. Therefore, MRI offers a noninvasive approach to serially monitor the progression of the disease. Compared with other imaging modalities, MRI appears to have the greatest potential for plaque characterization, through the use of multiple contrast weightings (e.g., T1, T2, and proton density). Here, we illustrate a standard procedure to image the aorta of atherosclerotic mice using noninvasive MRI.

Keywords: Aorta; Atherosclerosis; Magnetic resonance imaging (MRI); Plaque; apoE-KO mice.

MeSH terms

  • Animals
  • Aorta / metabolism
  • Aorta / pathology*
  • Aortic Diseases / genetics
  • Aortic Diseases / metabolism
  • Aortic Diseases / pathology*
  • Apolipoproteins E / deficiency
  • Apolipoproteins E / genetics
  • Atherosclerosis / genetics
  • Atherosclerosis / metabolism
  • Atherosclerosis / pathology*
  • Biopsy
  • Disease Models, Animal
  • Image Interpretation, Computer-Assisted
  • Magnetic Resonance Imaging*
  • Mice, Knockout
  • Plaque, Atherosclerotic*
  • Predictive Value of Tests
  • Reproducibility of Results

Substances

  • Apolipoproteins E