Bio-mediated synthesis, characterization and cytotoxicity of gold nanoparticles

Phys Chem Chem Phys. 2015 Nov 21;17(43):29014-9. doi: 10.1039/c5cp01619c.

Abstract

We report here a "green" approach for the synthesis of gold nanoparticles (GNPs) in which the Mentha piperita extract was applied for the bioreduction of chloroauric acid and the stabilization of the formed nanostructures. The obtained GNPs were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy (TEM). The reduction of gold ions with the plant extract leads to the production of nanoparticles with various shapes (spherical, triangular and hexagonal) and sizes (from 10 to 300 nm). The kinetics of the reaction was monitored and various conditions of the synthesis were investigated. As a result, we established protocols optimized towards the synthesis of nanospheres and nanoprisms of gold. The cytotoxic effect of the obtained gold nanoparticles was studied by performing MTT assay, which showed lower cytotoxicity of the biosynthesized GNPs compared to gold nanorods synthesized using the usual seed-mediated growth. The results suggest that the synthesis using plant extracts may be a useful method to produce gold nanostructures for various biological and medical applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival / drug effects
  • Chlorides / chemistry
  • Gold / chemistry*
  • Gold Compounds / chemistry
  • Green Chemistry Technology
  • HEK293 Cells
  • Humans
  • Kinetics
  • Mentha piperita / chemistry
  • Mentha piperita / metabolism
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / toxicity
  • Metal Nanoparticles / ultrastructure
  • Oxidation-Reduction
  • Particle Size
  • Plant Extracts / chemistry
  • Spectrophotometry, Ultraviolet

Substances

  • Chlorides
  • Gold Compounds
  • Plant Extracts
  • Gold
  • gold tetrachloride, acid