Expression of ricin B chain in Escherichia coli

K Hussain; C Bowler; L M Roberts; J M Lord

doi:10.1016/0014-5793(89)80568-x

Expression of ricin B chain in Escherichia coli

FEBS Lett. 1989 Feb 27;244(2):383-7. doi: 10.1016/0014-5793(89)80568-x.

Authors

K Hussain¹, C Bowler, L M Roberts, J M Lord

Affiliation

¹ Department of Biological Sciences, University of Warwick, Coventry, England.

PMID: 2646152
DOI: 10.1016/0014-5793(89)80568-x

Abstract

DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / genetics*
Genes
Plant Lectins
Plants, Toxic*
Recombinant Fusion Proteins / isolation & purification
Restriction Mapping
Ricin / genetics*
Ricin / isolation & purification
Ricinus / genetics*

Substances

Plant Lectins
Recombinant Fusion Proteins
Ricin