miR-425 inhibits melanoma metastasis through repression of PI3K-Akt pathway by targeting IGF-1

Biomed Pharmacother. 2015 Oct:75:51-7. doi: 10.1016/j.biopha.2015.08.010. Epub 2015 Sep 2.

Abstract

miR-425 is a potential tumor suppressor in cancer, but its role in melanoma is still unknown. We aim to investigate miR-425 expression in melanoma tissues and cell lines. Next, cell proliferation, cell cycle, apoptosis and metastasis will be studied using lentivirus-mediated gain-of-function studies. The predicted results are stable miR-425 inhibits cell proliferation and metastasis and induced cell apoptosis. It is predicted that IGF-1 is a potential target gene of miR-495 by bioinformatics analysis. Then luciferase assay analysis identifies IGF-1 as a new direct target gene of miR-425 and miR-425 inhibits melanoma cancer progression via IGF-1. Collectively, our findings suggested that miR-425 may function as a tumor suppressor in melanoma by targeting IGF-1.

Keywords: IGF-1; Melanoma; Metastasis; miR-425.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Apoptosis
  • Binding Sites
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Humans
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism*
  • Melanoma / enzymology*
  • Melanoma / genetics*
  • Melanoma / secondary
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Phosphatidylinositol 3-Kinase / metabolism*
  • Proto-Oncogene Proteins c-akt / metabolism*
  • RNA Interference
  • Signal Transduction
  • Skin Neoplasms / enzymology*
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / pathology
  • Time Factors
  • Transfection

Substances

  • 3' Untranslated Regions
  • IGF1 protein, human
  • MIRN425 microRNA, human
  • MicroRNAs
  • Insulin-Like Growth Factor I
  • Phosphatidylinositol 3-Kinase
  • Proto-Oncogene Proteins c-akt