Enhancing Pharmacokinetics, Tumor Accumulation, and Antitumor Efficacy by Elastin-Like Polypeptide Fusion of Interferon Alpha

Jin Hu; Guilin Wang; Xinyu Liu; Weiping Gao

doi:10.1002/adma.201503440

Enhancing Pharmacokinetics, Tumor Accumulation, and Antitumor Efficacy by Elastin-Like Polypeptide Fusion of Interferon Alpha

Adv Mater. 2015 Dec 2;27(45):7320-4. doi: 10.1002/adma.201503440. Epub 2015 Oct 14.

Authors

Jin Hu¹, Guilin Wang¹, Xinyu Liu¹, Weiping Gao¹

Affiliation

¹ Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.

PMID: 26463662
DOI: 10.1002/adma.201503440

Abstract

Genetic fusion of elastin-like polypeptide (ELP) to the C-terminus of interferon alpha (IFN) generates a well-defined IFN-ELP fusion protein with high yield and well-retained bioactivity. The fusion protein significantly enhances pharmacokinetics, tumor accumulation, and antitumor efficacy of interferon alpha in a murine cancer model.

Keywords: cancer therapy; elastin-like polypeptides; interferon; pharmacokinetics; protein delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / metabolism
Antineoplastic Agents / pharmacokinetics*
Antineoplastic Agents / therapeutic use
Circular Dichroism
Dynamic Light Scattering
Elastin / genetics
Elastin / metabolism
Escherichia coli / metabolism
Half-Life
Humans
Interferon-alpha / blood
Interferon-alpha / genetics
Interferon-alpha / metabolism*
Mice
Neoplasms / drug therapy
Peptides / genetics
Peptides / metabolism*
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / blood
Recombinant Fusion Proteins / pharmacokinetics*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tissue Distribution

Substances

Antineoplastic Agents
Interferon-alpha
Peptides
Recombinant Fusion Proteins
Elastin