Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

Karuppiah Bhuvaneshwari; Ananda Gokulanathan; Malayandi Jayanthi; Vaithiyanathan Govindasamy; Luigi Milella; Sungyoung Lee; Deok Chun Yang; Shanmugam Girija

doi:10.1016/j.foodchem.2015.07.136

Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

Food Chem. 2016 Mar 1:194:55-60. doi: 10.1016/j.foodchem.2015.07.136. Epub 2015 Jul 29.

Authors

Karuppiah Bhuvaneshwari¹, Ananda Gokulanathan², Malayandi Jayanthi¹, Vaithiyanathan Govindasamy¹, Luigi Milella³, Sungyoung Lee⁴, Deok Chun Yang⁵, Shanmugam Girija⁶

Affiliations

¹ Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India.
² Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India; Korean Ginseng Center and Ginseng Resource Bank, Kyung Hee University, Yongin 449-701, Republic of Korea.
³ Department of Science, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
⁴ Department of Computer Engineering, Kyung Hee University, Yongin 449-701, Republic of Korea.
⁵ Korean Ginseng Center and Ginseng Resource Bank, Kyung Hee University, Yongin 449-701, Republic of Korea. Electronic address: [email protected].
⁶ Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India. Electronic address: [email protected].

PMID: 26471526
DOI: 10.1016/j.foodchem.2015.07.136

Abstract

In this study Ocimum basilicum L. (OB) and Ocimum tenuiflorum L. (OT) in vitro culture standardisation for increasing eugenol distribution, in comparison to their respective field grown parts was carried out. Eugenol was quantified using an optimised HPLC method and its relation with the total phenolic content (TPC) was measured. In vitro grown leaves and somatic embryos, of both OB and OT were found to contain similar quantities of eugenol (85μg/g approximately), higher than OB and OT field-grown leaves (30.2μg/g and 25.1μg/g respectively). It was also determined that in vitro grown leaves were richer in TPC than the field-grown intact organs. Results demonstrated the prominence of in vitro cultures for eugenol extraction. This study underlines that important food flavouring metabolites (e.g. vanillin, vanillic acids) might be produced, via the eugenol pathway, in Ocimum species that may be a good potential source of eugenol.

Keywords: Eugenol; In vitro; Ocimum basilicum; Ocimum tenuiflorum; RP-HPLC; Total phenolic content.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Eugenol / chemistry*
In Vitro Techniques
Ocimum basilicum / chemistry*
Plant Leaves / chemistry*

Substances

Eugenol