Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

Vy Tran; Daniel S Poole; Justin J Jeffery; Timothy P Sheahan; Donald Creech; Aleksey Yevtodiyenko; Andrew J Peat; Kevin P Francis; Shihyun You; Andrew Mehle

doi:10.3390/v7102873

Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

Viruses. 2015 Oct 13;7(10):5319-27. doi: 10.3390/v7102873.

Authors

Vy Tran^{1

2}, Daniel S Poole³, Justin J Jeffery⁴, Timothy P Sheahan⁵, Donald Creech⁶, Aleksey Yevtodiyenko⁷, Andrew J Peat⁸, Kevin P Francis⁹, Shihyun You¹⁰, Andrew Mehle¹¹

Affiliations

¹ Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. [email protected].
² Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA. [email protected].
³ Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. [email protected].
⁴ Carbone Cancer Center, University of Wisconsin Madison, Madison, WI 53706, USA. [email protected].
⁵ GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. [email protected].
⁶ GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. [email protected].
⁷ PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. [email protected].
⁸ GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. [email protected].
⁹ PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. [email protected].
¹⁰ GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. [email protected].
¹¹ Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. [email protected].

Abstract

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.

Keywords: influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiviral Agents / therapeutic use
Disease Models, Animal
Female
Genes, Reporter
Influenza A virus / growth & development*
Luminescent Measurements / methods
Mice, Inbred BALB C
Multimodal Imaging / methods*
Optical Imaging / methods
Orthomyxoviridae Infections / drug therapy
Orthomyxoviridae Infections / pathology*
Orthomyxoviridae Infections / virology*
Staining and Labeling / methods
Treatment Outcome
Viral Load
Whole Body Imaging / methods*

Substances

Antiviral Agents

Abstract

Publication types

MeSH terms

Substances

Grants and funding