Abstract
The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.
Keywords:
BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering.
Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Chromosomes, Artificial, Bacterial / chemistry
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Chromosomes, Artificial, Bacterial / metabolism
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DNA, Complementary / genetics*
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DNA, Complementary / metabolism
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Drosophila melanogaster / genetics
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Drosophila melanogaster / metabolism
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Genetic Engineering / methods*
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Genomics / methods*
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Humans
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Molecular Imaging / methods*
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Staining and Labeling / methods
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Transgenes*
Substances
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DNA, Complementary
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Fluorescent Dyes