The utility of transposon mutagenesis for cancer studies in the era of genome editing

Gina M DeNicola; Florian A Karreth; David J Adams; Chi C Wong

doi:10.1186/s13059-015-0794-y

The utility of transposon mutagenesis for cancer studies in the era of genome editing

Genome Biol. 2015 Oct 19:16:229. doi: 10.1186/s13059-015-0794-y.

Authors

Gina M DeNicola¹, Florian A Karreth², David J Adams³, Chi C Wong^{4

5}

Affiliations

¹ Meyer Cancer Center, Weill Cornell Medical College, New York, NY, 10021, USA.
² Meyer Cancer Center, Weill Cornell Medical College, New York, NY, 10021, USA. [email protected].
³ Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1HH, UK.
⁴ Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1HH, UK. [email protected].
⁵ Department of Haematology, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK. [email protected].

Abstract

The use of transposons as insertional mutagens to identify cancer genes in mice has generated a wealth of information over the past decade. Here, we discuss recent major advances in transposon-mediated insertional mutagenesis screens and compare this technology with other screening strategies.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
DNA Transposable Elements*
Disease Progression
Drug Resistance, Neoplasm / genetics
Gene Expression Regulation, Neoplastic
Genes, Neoplasm*
Genome
Humans
Mice
Mutagenesis, Insertional / methods*
Neoplasm Metastasis
Neoplasms / genetics*

Substances

DNA Transposable Elements

Abstract

Publication types

MeSH terms

Substances

Grants and funding