Abstract
miRNAs are a group of small RNAs that have been reported to play a key role at each stage of tumorigenesis and are believed to have future practical value. We now demonstrate that Bim, which stimulates cell apoptosis, is obviously down-regulated in pancreatic cancer (PaC) tissues and cell lines. And Bim-related miR-24 is significantly up-regulated in PaC. The repressed expression of Bim is proved to be a result of miR-24, thus promoting cell growth of both cancer and vascular cells, and accelerating vascular ring formation. By using mouse tumor model, we clearly showed that miR-24 promotes tumor growth and angiogenesis by suppressing Bim expression in vivo. Therefore, a new pathway comprising miR-24 and Bim can be used in the exploration of drug-target therapy of PaC.
Keywords:
Bim; angiogenesis; miR-24; pancreatic cancer; tumorigenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis Regulatory Proteins / metabolism*
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Bcl-2-Like Protein 11
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Blotting, Western
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Cell Proliferation / genetics
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Fluorescent Antibody Technique
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Gene Expression Regulation, Neoplastic / genetics*
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Heterografts
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Humans
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Male
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Membrane Proteins / metabolism*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Neovascularization, Pathologic / genetics*
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Pancreatic Neoplasms / genetics
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Pancreatic Neoplasms / metabolism
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Pancreatic Neoplasms / pathology*
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Proto-Oncogene Proteins / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction* / physiology
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Transfection
Substances
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Apoptosis Regulatory Proteins
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BCL2L11 protein, human
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Bcl-2-Like Protein 11
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Bcl2l11 protein, mouse
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MIRN24 microRNA, human
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Membrane Proteins
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MicroRNAs
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Proto-Oncogene Proteins