Abstract
Plasmodium falciparum, strain FCR3, genomic DNA that encodes the SERA gene of P. falciparum was isolated and sequenced. The SERA gene coding region was interrupted by 3 introns, the largest number observed, so far, in any Plasmodium gene. Two SERA gene alleles, allele I and allele II, were identified in the FCR3 strain, while only allele I was found in the Honduras-1 strain. Allele I mRNA was abundant in vivo during the late trophozoite and schizont stages. Allele II mRNA was either not expressed, or it was labile.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Animals
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Antigens, Protozoan / genetics*
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Antigens, Protozoan / isolation & purification
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA
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Exons
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Genes*
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Introns
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Molecular Sequence Data
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Oligonucleotides / chemical synthesis
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Plasmodium falciparum / genetics*
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RNA Splicing
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Sequence Homology, Nucleic Acid
Substances
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Antigens, Protozoan
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Oligonucleotides
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RNA, Messenger
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DNA