Electrochemical immunosensor based on Pd-Au nanoparticles supported on functionalized PDDA-MWCNT nanocomposites for aflatoxin B1 detection

Anal Biochem. 2016 Feb 1:494:10-5. doi: 10.1016/j.ab.2015.10.008. Epub 2015 Oct 30.

Abstract

This paper reports a label-free electrochemical immunosensor for the determination of aflatoxin B1 (AFB1), which is based on a gold electrode modified by a biocompatible film of carbon nanotubes/poly(diallyldimethylammoniumchloride)/Pd-Au nanoparticles (CNTs/PDDA/Pd-Au). The nanocomposite was characterized by transmission electron microscopy and the electrochemical behavior of modified electrodes was investigated by cyclic voltammetry. The CNTs/PDDA/Pd-Au nanocomposites film showed good electron transfer ability, which ensured high sensitivity to detect AFB1 in a range from 0.05 to 25 ng mL(-1) with a detection limit of 0.03 ng mL(-1) obtained at 3σ (where σ is the standard deviation of the blank solution, n = 10). The proposed immunosensor provides a simple tool for AFB1 detection. This strategy can be extended to any other antigen detection by using the corresponding antibodies.

Keywords: Aflatoxin B1; Carbon nanotubes; Immunosensors; Pd–Au nanoparticles; Poly(diallyldimethylammoniumchloride).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin B1 / analysis*
  • Aflatoxin B1 / immunology
  • Antibodies, Immobilized / immunology
  • Biosensing Techniques
  • Electrochemical Techniques*
  • Electrodes
  • Food Contamination / analysis
  • Gold / chemistry
  • Limit of Detection
  • Nanocomposites / chemistry*
  • Nanotubes, Carbon / chemistry*
  • Oryza / chemistry
  • Palladium / chemistry
  • Polyethylenes / chemistry*
  • Quaternary Ammonium Compounds / chemistry*

Substances

  • Antibodies, Immobilized
  • Nanotubes, Carbon
  • Polyethylenes
  • Quaternary Ammonium Compounds
  • poly-N,N-dimethyl-N,N-diallylammonium chloride
  • Palladium
  • Gold
  • Aflatoxin B1