Abstract
A silicon-based microarray system was constructed to discover the affinity peptides and to distinguish the specific peptides from a high throughput library. Using a color-encoding strategy, in situ peptide distinguishing between HER1 ligands and HER2 ligands was achieved. Novel affinity peptide sequences H1P (HER1 ligand) and H2P (HER2 ligand) were determined with nmol affinity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Cell Line, Tumor
-
Equipment Design
-
ErbB Receptors / metabolism*
-
Humans
-
Ligands
-
Molecular Docking Simulation
-
Molecular Sequence Data
-
Peptide Library*
-
Peptides / chemistry
-
Peptides / metabolism*
-
Protein Array Analysis / instrumentation*
-
Receptor, ErbB-2 / metabolism*
Substances
-
Ligands
-
Peptide Library
-
Peptides
-
EGFR protein, human
-
ERBB2 protein, human
-
ErbB Receptors
-
Receptor, ErbB-2