Chrysin suppresses human CD14(+) monocyte-derived dendritic cells and ameliorates experimental autoimmune encephalomyelitis

J Neuroimmunol. 2015 Nov 15:288:13-20. doi: 10.1016/j.jneuroim.2015.08.017. Epub 2015 Sep 1.

Abstract

Chrysin, a naturally flavonoid of plant, has various biological activities. However, the effects of chrysin on dendritic cells (DCs) and multiple sclerosis (MS) remain unknown. In this study, we demonstrate that chrysin inhibited human DC differentiation, maturation, function and the expression of the Th1 cells polarizing cytokines IFN-γ and IL-12p35 form DCs. In addition, chrysin ameliorated experimental autoimmune encephalomyelitis (EAE), an animal model of MS, by reducing CNS inflammation and demyelination. Furthermore, chrysin suppressed DCs and Th1 cells in the EAE mice. Taken together, chrysin exerts anti-inflammatory and immune suppressive effects, and suggests a possible therapeutic application of chrysin in MS.

Keywords: Chrysin; Dendritic cells; Experimental autoimmune encephalomyelitis; Inflammation; Multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / immunology
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Endocytosis / drug effects
  • Female
  • Flavonoids / pharmacology*
  • Fluorescent Antibody Technique
  • Humans
  • Immunosuppressive Agents / pharmacology*
  • Lipopolysaccharide Receptors / immunology
  • Lymphocyte Activation / drug effects
  • Lymphocyte Culture Test, Mixed
  • Mice
  • Mice, Inbred C57BL
  • Real-Time Polymerase Chain Reaction

Substances

  • Flavonoids
  • Immunosuppressive Agents
  • Lipopolysaccharide Receptors
  • chrysin