1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state

Biomol NMR Assign. 2016 Apr;10(1):157-61. doi: 10.1007/s12104-015-9657-4. Epub 2015 Nov 4.

Abstract

Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins with a tetrapyrrole (bilin) chromophore that belong to the phytochrome superfamily. Like phytochromes, CBCRs photoconvert between two photostates with distinct spectral properties. NpR6012g4 from Nostoc punctiforme is a model system for widespread CBCRs with conserved red/green photocycles. Atomic-level structural information for the photoproduct state in this subfamily is not known. Here, we report NMR backbone chemical shift assignments of the light-activated state of NpR6012g4 (BMRB no. 26577) as a first step toward determining its atomic resolution structure.

Keywords: CBCR; Cyanobacteria; Cyanobacteriochrome; NMR; Photoreceptor; Phycocyanobilin; Phytochrome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Absorption, Radiation*
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Color
  • Nostoc*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Photoreceptors, Microbial / chemistry*
  • Photoreceptors, Microbial / metabolism*

Substances

  • Bacterial Proteins
  • Photoreceptors, Microbial