NIR-to-NIR Two-Photon Scanning Laser Microscopy Imaging of Single Nanoparticles Doped by Yb(III) Complexes

Chemphyschem. 2016 Jan 4;17(1):128-35. doi: 10.1002/cphc.201500814. Epub 2015 Nov 23.

Abstract

The photophysical and nonlinear optical properties of water-soluble chromophore-functionalised tris-dipicolinate complexes [LnL3](3-) (Ln=Yb and Nd) are thoroughly studied, revealing that only the Yb(III) luminescence can be sensitized by a two-photon excitation process. The stability of the complex in water is strongly enhanced by embedding in dispersible organosilicate nanoparticles (NPs). Finally, the spectroscopic properties of [NBu4]3 [YbL3] are studied in solution and in the solid state. The high brightness of the NPs allows imaging them as single objects using a modified two-photon microscopy setup in a NIR-to-NIR configuration.

Keywords: fluorescence; lanthanide complexes; nanoparticles; two-photon microscopy; ytterbium.

MeSH terms

  • Coordination Complexes / chemical synthesis
  • Coordination Complexes / chemistry
  • Coordination Complexes / radiation effects*
  • Light
  • Microscopy, Confocal / methods*
  • Nanoparticles / chemistry
  • Nanoparticles / radiation effects*
  • Picolinic Acids / chemical synthesis
  • Picolinic Acids / chemistry
  • Picolinic Acids / radiation effects*
  • Spectroscopy, Near-Infrared
  • Ytterbium / chemistry*

Substances

  • Coordination Complexes
  • Picolinic Acids
  • Ytterbium