Adaptation of microcystin thiol derivatization for matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis

Jonathan Puddick; Christopher O Miles; Michèle R Prinsep

doi:10.1016/j.toxicon.2015.11.006

Adaptation of microcystin thiol derivatization for matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis

Toxicon. 2016 Jan:109:13-7. doi: 10.1016/j.toxicon.2015.11.006. Epub 2015 Nov 10.

Authors

Jonathan Puddick¹, Christopher O Miles², Michèle R Prinsep³

Affiliations

¹ Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand. Electronic address: [email protected].
² Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106 Oslo, Norway; Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway.
³ Department of Chemistry, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand.

PMID: 26559614
DOI: 10.1016/j.toxicon.2015.11.006

Abstract

Thiol derivatization of microcystins is an emerging technique for simplifying mass spectrometric analysis of microcystins in complex matrices and for distinguishing between Mdha-/Dhb-containing microcystins. The present protocol is not compatible with MALDI-TOF mass spectrometry, but use of sodium carbonate buffer and desalting with ZipTips yielded no loss in sensitivity. Use of ammonium carbonate buffer followed by dilution in MALDI matrix resulted in a small loss of sensitivity but allowed accurate determination of reaction rates.

Keywords: MALDI-TOF mass spectrometry; Microcystin; Thiol derivatization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Microcystins / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sulfhydryl Compounds / analysis*

Substances

Microcystins
Sulfhydryl Compounds