A FRET-based ratiometric two-photon fluorescent probe for dual-channel imaging of nitroxyl in living cells and tissues

Chem Commun (Camb). 2016 Jan 14;52(4):733-6. doi: 10.1039/c5cc08695g.

Abstract

A FRET-based two-photon fluorescent probe, , which exhibited a fast and high selective ratiometric response to nitroxyl, was first proposed. was successfully applied to two-photon dual-channel imaging of nitroxyl in living cells and tissues with less cross-talk between channels and satisfactory deep-tissue imaging depth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells / ultrastructure*
  • Diagnostic Imaging
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • HeLa Cells
  • Humans
  • Nitrogen Oxides / analysis
  • Nitrogen Oxides / chemistry*
  • Photons

Substances

  • Fluorescent Dyes
  • Nitrogen Oxides
  • nitroxyl