Abstract
We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
5-Methylcytosine / chemistry*
-
5-Methylcytosine / metabolism
-
Animals
-
Cytosine / analogs & derivatives*
-
Cytosine / chemistry
-
Cytosine / metabolism
-
DNA / chemistry*
-
DNA / metabolism
-
DNA Methylation
-
Mammals
-
Mass Spectrometry
-
Oxidation-Reduction
-
RNA / chemistry*
-
RNA / metabolism
Substances
-
5-formylcytosine
-
5-hydroxymethylcytosine
-
RNA
-
5-Methylcytosine
-
Cytosine
-
DNA