Abstract
Here, we present a sub-μm multimodal approach to image essential elements in Caenorhabditis elegans. A combination of chemical imaging technologies reveals total metal concentration, chemical state and the protein to which an element is associated. This application of distinct yet complementary chemical imaging techniques provided unique insight into essential and trace elements at the subcellular level.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Caenorhabditis elegans Proteins
-
Caenorhabditis elegans* / anatomy & histology
-
Caenorhabditis elegans* / chemistry
-
Caenorhabditis elegans* / metabolism
-
Image Processing, Computer-Assisted / methods*
-
Metals / chemistry*
-
Metals / metabolism*
-
Molecular Imaging / methods*
-
Spectrometry, X-Ray Emission / methods*
Substances
-
Caenorhabditis elegans Proteins
-
Metals