Staphylokinase and ABO group phenotype: new players in Staphylococcus aureus implant-associated infections development

Future Microbiol. 2015;10(12):1929-38. doi: 10.2217/fmb.15.112. Epub 2015 Nov 23.

Abstract

Aim: To identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI).

Materials & methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods.

Results: Staphylokinase gene was associated with the occurrence of S. aureus IAI. Patients' ABO blood group phenotype was associated with IAI S. aureus genetic background. CC8 S. aureus strains produce more biofilm than others and carry particular alleles of bbp gene.

Conclusion: This study identifies some predictive markers for S. aureus IAI.

Keywords: Staphylococcus aureus; biofilm; blood group; bone and joint infection; bone binding sialoprotein; microarrays; staphylokinase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System / metabolism*
  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Genotype
  • Host-Pathogen Interactions*
  • Humans
  • Male
  • Metalloendopeptidases / metabolism*
  • Microarray Analysis
  • Middle Aged
  • Multilocus Sequence Typing
  • Phenotype
  • Prosthesis-Related Infections / microbiology*
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / isolation & purification*
  • Staphylococcus aureus / pathogenicity
  • Young Adult

Substances

  • ABO Blood-Group System
  • Metalloendopeptidases
  • auR protein, Staphylococcus aureus