A new genotype of border disease virus with implications for molecular diagnostics

Arch Virol. 2016 Feb;161(2):471-7. doi: 10.1007/s00705-015-2696-4. Epub 2015 Nov 23.

Abstract

Border disease virus (BDV) is a (+) single-stranded RNA pestivirus affecting mainly sheep and goats worldwide. Genetic typing of BDV has led to the identification of at least seven major genotypes. This study reports the detection of a BDV strain from a goat in northwestern Italy during routine investigations. Sequence analysis revealed mutations in the 5'-UTR of the virus with implications for BDV molecular diagnostics. Moreover, subsequent phylogenetic analysis based on the combined 5'-UTR and Npro/partial C genes, showed divergence from known BDV genotypes, revealing the detection of a novel pestivirus group, for which we propose the name BDV genotype 8.

Keywords: Border disease; Genotype; Goat; Italy; Pestivirus; Phylogenetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions*
  • Animals
  • Border Disease / diagnosis*
  • Border Disease / virology*
  • Border disease virus / classification*
  • Border disease virus / genetics*
  • Border disease virus / isolation & purification
  • Cluster Analysis
  • Genotype*
  • Goats
  • Italy
  • Molecular Sequence Data
  • Mutation
  • Pathology, Molecular
  • Phylogeny
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA

Substances

  • 5' Untranslated Regions
  • RNA, Viral

Associated data

  • GENBANK/KT072634
  • GENBANK/KT072635