In Vitro Effect of Laser-Induced Hydrodynamics on Cancer Cells

Bull Exp Biol Med. 2015 Nov;160(1):155-9. doi: 10.1007/s10517-015-3117-0. Epub 2015 Nov 24.

Abstract

We studied the effect of laser-induced hydrodynamic on viability of Colo-26 murine colon carcinoma cells in vitro. Laser-induced hydrodynamics was generated by a laser (λ=1.56 μ, power 3 W, 5 min exposure); to this end, the fiber end was submersed into a buffer above the cell monolayer. It was found that laser-induced hydrodynamics destructed the monolayer at standoff distances of between the working end of the laser fiber to cell monolayer of 1 and 5 mm and triggers apoptotic and necrotic death in remaining cells at a distance of 4 mm from the emitter.

Keywords: apoptosis; laser radiation; laser-induced hydrodynamics; necrosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Carcinoma / pathology*
  • Cell Line, Tumor
  • Colonic Neoplasms / pathology*
  • Fiber Optic Technology / instrumentation
  • Fiber Optic Technology / methods*
  • Hydrodynamics*
  • In Vitro Techniques
  • Laser Therapy / instrumentation
  • Laser Therapy / methods
  • Lasers*
  • Mice
  • Microbubbles
  • Necrosis
  • Temperature
  • Video Recording