Functional imaging of the nonhuman primate Placenta with endogenous blood oxygen level-dependent contrast

Magn Reson Med. 2016 Nov;76(5):1551-1562. doi: 10.1002/mrm.26052. Epub 2015 Nov 24.

Abstract

Purpose: To characterize spatial patterns of T2* in the placenta of the rhesus macaque (Macaca mulatta), to correlate these patterns with placental perfusion determined using dynamic contrast-enhanced MRI (DCE-MRI), and to evaluate the potential for using the blood oxygen level-dependent effect to quantify placental perfusion without the use of exogenous contrast reagent.

Methods: MRI was performed on three pregnant rhesus macaques at gestational day 110. Multiecho spoiled gradient echo measurements were used to compute maps of T2*. Spatial maxima in these maps were compared with foci of early enhancement determined by DCE-MRI.

Results: Local maxima in T2* maps were strongly correlated with spiral arteries identified by DCE-MRI, with mean spatial separations ranging from 2.34 to 6.11 mm in the three animals studied. Spatial patterns of R2* ( = 1/ T2*) within individual placental lobules can be quantitatively analyzed using a simple model to estimate fetal arterial oxyhemoglobin concentration [Hbo,f] and a parameter viPS/Φ, reflecting oxygen transport to the fetus. Estimated mean values of [Hbo,f] ranged from 4.25 mM to 4.46 mM, whereas viPS/Φ ranged from 2.80 × 105 cm-3 to 1.61 × 106 cm-3 .

Conclusions: Maternal spiral arteries show strong spatial correlation with foci of extended T2* observed in the primate placenta. A simple model of oxygen transport accurately describes the spatial dependence of R2* within placental lobules and enables assessment of placental function and oxygenation without requiring administration of an exogenous contrast reagent. Magn Reson Med 76:1551-1562, 2016. © 2015 International Society for Magnetic Resonance in Medicine.

Keywords: BOLD MRI; DCE-MRI; Placenta; Pregnancy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Contrast Media / metabolism
  • Female
  • Humans
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Macaca mulatta
  • Magnetic Resonance Imaging / methods*
  • Oxygen / blood*
  • Placenta / blood supply
  • Placenta / diagnostic imaging*
  • Placenta / physiology*
  • Placental Circulation / physiology*
  • Pregnancy
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Contrast Media
  • Oxygen